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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was uncovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two additional splice variants ended up documented but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically incorporates the epitope regarded from the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to guarantee the very best benefits are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is certain.
Goal Details Cyclin E belongs towards the really conserved cyclin family, whose associates are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which lead for the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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